Tang, MatthewQiu, HaoRoberts, SeleneLi, GuoliSu, RongMartin-Fernandez, Marisa LClarke, David T.Liu, ShugangLiu, XiaojieWang, Lin2025-10-062025-10-062025https://edata.stfc.ac.uk/handle/edata/982https://doi.org/10.5286/edata/9501. Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data. Used for axial drift and lateral drift quantification. 2. Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a commercial microscope. .tif files for images, .csv files for localization data 3. STORM image series of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 and localisation data, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data 4. STORM image series of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 and localisation data, acquired with a commercial microscope. .tif files for images, .csv files for localization data 5. STORM image series of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a homebuilt microscope. tif files for images, .csv files for localization data 6. STORM image series of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a commercial microscope. .tif files for images, .csv files for localization dataenCreative Commons Attribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/Dataset