Tang, MatthewQiu, HaoRoberts, SeleneLi, GuoliSu, RongMartin-Fernandez, Marisa L.Clarke, David T.Liu, ShugangLiu, XiaojieWang, Lin2025-05-142025-05-142025https://edata.stfc.ac.uk/handle/edata/974https://doi.org/10.5286/edata/942This dataset contains: 1) Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data 2) Five image series' of fluorescent beads and their subpixel localisations in each frame, acquired with a commercial microscope. .czi files for images, .csv files for localization data 3) STORM image series of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 and localisation data, acquired with a homebuilt microscope. .tif files for images, .csv files for localization data 4) STORM image series of HeLa cells with alpha-tubulin immunolabelled with antibodies conjugated to Alexa Fluor 647 and localisation data, acquired with a commercial microscope. .czi files for images, .csv files for localization data 5) STORM image series of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a homebuilt microscope. tif files for images, .csv files for localization data 6) STORM image series of U2OS cells with Nup96-SNAP-AF647 and localisation data acquired with a commercial microscope. .czi files for images, .csv files for localization dataenCreative Commons Attribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/Single-molecule localization microscopyStochastic optical reconstruction microscopySuper-resolution imagingSMLMSTORMDrift-free SMLMReinforced Optical Cage SystemCollection