Data for: The use of FLIM for characterising chromosomes and their structure in response to low-dose X-ray irradiation

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dc.contributor.authorBotchway, Stanley
dc.contributor.authorYusuf, Mohammed
dc.contributor.authorBerger, Sarah
dc.contributor.authorMohammed, Rosie
dc.contributor.authorBhartiya, Archana
dc.contributor.authorPhengchat, Rinyaporn
dc.contributor.authorBarnard, Stephen
dc.contributor.authorBateman, Benji
dc.contributor.authorRobinson, Ian
dc.contributor.funderUKRI
dc.date.accessioned2025-12-12T09:47:34Z
dc.date.issued2025-12-12
dc.description.abstractThe files in this repository are the raw and analysed data presented in the paper titled above. The experimental conditions are highlighted in the following published articles. We applied these methods to different DNA stains bound to chromosomes where only DAPI led to a clear lifetime difference between the chromosome arms (p,q). Our data showed that chromosomes of cells irradiated with 0.1 Gy and 1 Gy did not show a significant change in lifetimes. These findings indicate that DAPI FLT may be a useful tool to measure chromosomal structural changes and further suggests that chromosomes undergo distinct structural changes at the pericentromeric region following low-dose irradiation. Note: this deposit is continued by: https://doi.org/10.5286/edata/962
dc.description.sponsorshipBBSRC grant BB/H022597/1
dc.identifier.urihttps://edata.stfc.ac.uk/handle/edata/991
dc.identifier.urihttps://doi.org/10.5286/edata/959
dc.language.isoen
dc.relation.iscontinuedbyhttps://doi.org/10.5286/edata/962
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.otherChromosomes
dc.subject.otherFluorescence Lifetime Imaging Microscopy (FLIM)
dc.subject.otherConfocal Imaging
dc.subject.otherHeLa Cells, T Cells,
dc.subject.otherX-ray Irradiation
dc.subject.otherIonising Radiation
dc.titleData for: The use of FLIM for characterising chromosomes and their structure in response to low-dose X-ray irradiation
dc.typeCollection

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