Extension to Data for: The use of FLIM for characterising chromosomes and their structure in response to low-dose X-ray irradiation

Abstract

more files and extension to - https://doi.org/10.5286/edata/959 .In this repository are the raw and analysed data presented in the paper titled above. The experimental conditions are highlighted in the following published articles. 1. Botchway SW, Parker AW, Bisby RH, Crisostomo AG. Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation. Microscopy Research and Technique [Internet]. 2008 [cited 2025 Sep 9];71(4):267–73. Available from: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jemt.20548 2. Ahmed A, Schoberer J, Cooke E, Botchway SW. Multicolor FRET-FLIM Microscopy to Analyze Multiprotein Interactions in Live Cells. Methods in molecular biology [Internet]. 2020 Dec 10 [cited 2025 Sep 9];287–301. Available from: https://link.springer.com/protocol/10.1007/978-1-0716-1126-5_16 3. Stubbs CD, Botchway SW, Slater SJ, Parker AW. The use of time-resolved fluorescence imaging in the study of protein kinase C localisation in cells. BMC Cell Biology [Internet]. 2005 Apr 26 [cited 2025 Sep 9];6(1). Available from: https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/1471-2121-6-22 4. Estandarte AK, Botchway S, Lynch C, Yusuf M, Robinson I. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes. Scientific Reports [Internet]. 2016 Aug 16 [cited 2025 Sep 9];6(1). Available from: https://www.nature.com/articles/srep31417 5. Berger SL, Phengchat R, Botchway SW, Yusuf M. Optimizing chromosome yield: a comparative analysis of harvesting, preparation and waste recovery methods. BioTechniques [Internet]. 2025 Jun 3 [cited 2025 Sep 9];77(5-6):245–56. Available from: https://www.tandfonline.com/doi/full/10.1080/07366205.2025.2536438?src=exp-la

Note: this deposit continues: https://doi.org/10.5286/edata/959